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room temperature rt  (Proteintech)


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    Structured Review

    Proteintech room temperature rt
    Room Temperature Rt, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/room+temperature+rt/GAPDH+Monoclonal+antibody/pm41782112-112-11-24
    Average 98 stars, based on 10433 article reviews
    room temperature rt - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: FADD regulates adipose inflammation, adipogenesis, and adipocyte survival
    Article Snippet: Equal amounts of proteins from each sample were loaded for electrophoresis (Bio-Rad). .. Proteins were separated by SDS–PAGE, transferred to a polyvinylidene fluoride membrane (Millipore Corporation), incubated in blocking buffer (5% fat-free milk in TBS) for 1 h at room temperature (RT), then incubated with primary antibodies (Anti-GFP, 50430-2-AP and Anti-HSP90, 13171-1-AP from Proteintech; Anti-FLAG, sab4301135 from SIGMA; Anti-RIPK1, 3493, Anti-RIPK3, 10188, Anti-BCL2, 15071 and Anti- Cleaved-Caspase-3, 9664 from Cell Signaling Technology) in blocking buffer overnight at 4 °C. ..

    Incubation:

    Article Title: FADD regulates adipose inflammation, adipogenesis, and adipocyte survival
    Article Snippet: Equal amounts of proteins from each sample were loaded for electrophoresis (Bio-Rad). .. Proteins were separated by SDS–PAGE, transferred to a polyvinylidene fluoride membrane (Millipore Corporation), incubated in blocking buffer (5% fat-free milk in TBS) for 1 h at room temperature (RT), then incubated with primary antibodies (Anti-GFP, 50430-2-AP and Anti-HSP90, 13171-1-AP from Proteintech; Anti-FLAG, sab4301135 from SIGMA; Anti-RIPK1, 3493, Anti-RIPK3, 10188, Anti-BCL2, 15071 and Anti- Cleaved-Caspase-3, 9664 from Cell Signaling Technology) in blocking buffer overnight at 4 °C. ..

    Article Title: PACAP38 synergizes with irradiation to suppress the proliferation of multiple cancer cells via regulating SOX6/Wnt/β-catenin signaling
    Article Snippet: .. At last, they were further incubated with HRP-conjugated secondary antibodies at room temperature (RT) for 1 h. The primary antibodies listed below were employed: anti-SOX6 (Proteintech, United States), anti-β-catenin (CST, United States), anti-LEF1 (CST, United States), anti-TCF1/TCF7 (CST, United States), anti-MMP7 (CST, United States), anti-cJUN (CST, United States), anti-c-myc (CST, United States), anti-CD44 (CST, United States), anti-Tubulin (CST, United States), anti-GAPDH (CST, UUnited States). .. The Acetyl-Histone Antibody Sampler Kit (CST, United States) was used to evaluate the acetylation states of histones H2A, H2B, H3, and H4.

    Article Title: A partial deletion of the Tardbp 3’UTR affects TDP-43 regulation and leads to motor dysfunction in mice
    Article Snippet: Protein samples were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-rad, Hercules, CA, USA). .. Membranes were blocked overnight in 5% skimmed milk (Wako, Osaka, Japan) and subsequently incubated at room temperature (RT) for 1 h with primary antibodies: TDP-43 polyclonal antibody (1:1,000, 10782-2-AP, Proteintech), anti-α-Tubulin antibody (1:5,000, T9026, Sigma). .. After washing in 0.05% Tween in TBS, the membranes were incubated for 1 h at RT with secondary antibodies: anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (1:5,000, G21040 , ThermoFisher Scientific), and anti-Rabbit IgG H&L (HRP) (1:5,000, ab6721, Abcam, Cambridge, UK).

    Article Title: TFAP2A regulates SGPP2 transcription to promote lipid accumulation and activate the Wnt/β-catenin signaling pathway to promote malignant progression in lung adenocarcinoma.
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (ISEQ00010, Millipore, Billerica, MA, USA) via a wet transfer. .. Membranes were blocked with 5% skim milk in TBST buffer at room temperature (RT) for 1 hour, then incubated with primary antibodies (GAPDH: 60004-1-Ig, Proteintech, 1:5000; β-actin: 66009-1-Ig, Proteintech, 1:5000; SGPP2: 102-23119, Raybiotech, 1:400; TFAP2A: 13019-3-AP, Proteintech, 1:4000; PCNA: 10205-2-AP, Proteintech, 1:5000; CyclinD1: 26939-1-AP, Proteintech, 1:2000; CyclinB1: 55004-1-AP, Proteintech, 1:1000; MMP9: AF5228, Affinity, 1:1000; E-cadherin: 20874-1-AP, Proteintech, 1:10000; N-cadherin: 22018-1-AP, Proteintech, 1:2000; Vimentin: 10366-1-AP, Proteintech, 1:5000; β-catenin: PT0300R, Immunoway, 1:2000; GSK3β: YM1273, Immunoway, 1:1000; p-GSK3β (Ser9): PT0078R, Immunoway, 1:1000; Histone H3: 17168-1-AP, Proteintech, 1:5000; SREBP1: 14088-1-AP, Proteintech, 1:2000; FASN: PT0554R, Immunoway, 1:4000; SCD1: YN2991, Immunoway, 1:1000; SGPP1: YN4019, Immunoway, 1:1000; SKI1: YT4309, Immunoway, 1:1000) overnight at 4°C. .. The next day, the membranes were incubated with goat gnti-rabbit or mouse IgG (H+L) (BS13278/BS12478, Bioworld; 1:20000) at RT for 1 hour.

    Article Title: The modified 6-chromanol SUL-238 protects against accelerated vascular aging in vascular smooth muscle <i>Ercc1</i>-deficient mice
    Article Snippet: When the required separation was reached, proteins were transferred to membranes (Trans-Blot Turbo 0.2 μm PVDF Transfer Pack, Bio-Rad) with the Trans-Blot Turbo Transfer System (Bio-Rad). .. Membranes were then blocked with 5% [w/v] milk or BSA for 2 h at room temperature (RT), followed by overnight incubation at 4 °C in primary antibody [GAPDH (1:10,000; 10494-1-AP, Proteintech); MMP2 (1:500, #87809, Cell Signaling), MMP9 (1:100, sc-393859, Santa-Cruz), p21 (1:200; sc6246, Santa-Cruz), p16 (1:500; 51-1325GR, BD Pharmingen)]. .. On the next day, the membranes were incubated for 1 h at RT in the secondary antibody [goat anti-mouse- or goat anti-rabbit-HRP conjugate (Bio-Rad, 1:3,000)], and visualized with Clarity Western ECL substrate (Bio-Rad) following the manufacturer’s instructions using an Amersham Al600.

    Blocking Assay:

    Article Title: FADD regulates adipose inflammation, adipogenesis, and adipocyte survival
    Article Snippet: Equal amounts of proteins from each sample were loaded for electrophoresis (Bio-Rad). .. Proteins were separated by SDS–PAGE, transferred to a polyvinylidene fluoride membrane (Millipore Corporation), incubated in blocking buffer (5% fat-free milk in TBS) for 1 h at room temperature (RT), then incubated with primary antibodies (Anti-GFP, 50430-2-AP and Anti-HSP90, 13171-1-AP from Proteintech; Anti-FLAG, sab4301135 from SIGMA; Anti-RIPK1, 3493, Anti-RIPK3, 10188, Anti-BCL2, 15071 and Anti- Cleaved-Caspase-3, 9664 from Cell Signaling Technology) in blocking buffer overnight at 4 °C. ..



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